P16-creert2-tdtomato mouse model p16-tdtomato
WebFigure Legend Snippet: Single-cell RNA sequencing (scRNA-seq) analysis of cardiac hypertrophy in a mouse model of pressure overload. A Schematic depicting the pipeline of transverse aortic constriction (TAC) surgery and tissue harvesting of Cd34 -CreERT2; Rosa26-tdTomato mouse for scRNA-seq. Heart tissue was harvested 0, 4 and 12 … WebSep 18, 2024 · In this mouse model, p16 high cells are labelled with tdTomato (red fluorescence) in vivo by tamoxifen administration. (*2) p16 high cells Cells expressing …
P16-creert2-tdtomato mouse model p16-tdtomato
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WebFig. 2 CreERT2-mediated recombination in the small intestine of Lgr5 CreERT2 /+; Rosa26 tdTomato/+ mouse. TdTomato (red) expression can be detected in the mucous membrane epithelial cells and crypt cells of small intestine derived from Lgr5 CreERT2 /+; Rosa26 tdTomato/+ mouse. EGFP (green) expression can not be observed. Webp16INK4a-tdTomato, p16-tdTomato, p16-tdTom, p16-Tomato, p16INK4a-Tomato. Strain Information Expand All. Gene Details [Including genotyping protocols] Cdkn2a tm4Nesh. Name: cyclin-dependent kinase ... 1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at ...
Web图1. TdTomato的打靶策略[1] 这种鼠在正常情况下由于3×stop的存在并不能表达红色荧光,当与Cre鼠或TAM激活的CreERT2鼠交配后,产生的后代由于Cre重组酶切割loxp位点之间的序列将3×stop切除,TdTomato得到表达,就可以在Cre或CreERT2表达的组织中发出红色荧光,指示基因的位置。 WebJul 6, 2024 · Figure 1 Design and generation of the inducible hCD68-CreERT2 model.(A) Presence of Cre recombinase and tdTomato genes was confirmed by extracting DNA from ear notches, a standard PCR amplification and running side-by-side on an agarose gel for all genotypes available for het x het breeding strategy (see methods for details).(B) …
WebKeratin 16 (KRT16 in human, Krt16 in mouse), a type I intermediate filament protein, is constitutively expressed in epithelial appendages and is induced in the epidermis upon … Web007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre …
WebJun 26, 2024 · A novel Tmem119-tdTomato reporter mouse model for studying microglia in the central nervous system. Brain Behav Immun. 2024 Jan;83:180-191. Epub 2024 Oct 8 PubMed. Kaiser T, Feng G. Tmem119-EGFP and Tmem119-CreERT2 transgenic mice for labeling and manipulating microglia. bioRχiv. May 19, 2024
Web033052 B6;129- Procr tm1(cre/ERT2)Yaz /J Procr CreERT2-IRES-tdTomato are knockin/knockout mice with an inducible cre /ERT2 fusion gene followed by a tdTomato reporter inserted into the ATG codon of the protein C receptor ( Procr ). These mice can be used to identify multipotent mammary stem cells (MaSCs) in the basal layer of the … imisystems.comWebMethods: A Gcg CreERT2 knockin mouse, which expresses a tamoxifen-inducible form of Cre from the endogenous preproglucagon (Gcg) gene locus, was generated by … imitate amusingly crossword clueWebSep 18, 2024 · A research team generated a p16-Cre ERT2 -tdTomato mouse model to characterize in vivo p16 high cells at the single-cell level. They found tdTomato-positive … imitate an owl crosswordWebAug 28, 2024 · The p16 CreERT2 tdTomato mouse model also demonstrated that p16 high cells were detectable in the liver, and that they were enriched with aging. The … list of regional banks under pressureimitando fotos kimberly loaizaWeb033052 B6;129- Procr tm1(cre/ERT2)Yaz /J Procr CreERT2-IRES-tdTomato are knockin/knockout mice with an inducible cre /ERT2 fusion gene followed by a tdTomato … list of regal entertainment group theatersWebDec 24, 2024 · We generated transgenic mice that specifically expressed tdTomato along with FAP promoter activity. Coculturing a mouse gastric cancer cell line and FAP-tdTomato transgenic mouse-derived fibroblasts showed that tdTomato expression was elevated in the cocultured fibroblasts. imitas toestemmingsformulier