Hoechst 33342 toxicity
NettetPage 2/6 Safety Data Sheet acc. to OSHA HCS Printing date 01/14/2024 Revision date 01/14/2024 Trade name: Hoechst 33342 (hydrochloride) (Contd. from page 1) NettetBisbenzimide ( Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. [1] Several related chemical compounds are used for similar purposes and are …
Hoechst 33342 toxicity
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Nettet18. jun. 2024 · The procedure was successfully tested for the analysis of cytotoxicity. The method is suitable for the quantification of cells exhibiting low metabolic activity including senescent cells. The... Nettet12. apr. 2024 · Nuclei were stained with Hoechst 33342 (1/1000). Anti-endomucin antibody (sc-65495, Santa Cruz, diluted 1/200) was used to stain vessels in bone marrow and spleen.
NettetHoechst dyes are generally preferred for live cell staining over DAPI because they are less toxic and more cell permeant. Biotium offers both Hoechst 33342 and Hoechst … NettetHoechst 33342 Binding. Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell …
NettetHoechst 33342 induced reactive oxygen species and impaired expression of cytochrome c oxidase subunit 1 leading to cell death in irradiated human cancer cells Oxidative stress … NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye —blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient —provided as an easy-to-use Hoechst dye solution (20 mM) • Cellular imaging —cell-permeable dye is effective for fixed-cell and live-cell staining. • Counterstain —ideal for use ...
Nettet18. jun. 2016 · The chemical study on the seeds of Caesalpinia sappan led to the isolation of five new cassane diterpenoids, phanginins R‒T (1–3) and caesalsappanins M and N (4 and 5), together with seven known compounds 6–12. Their structures were elucidated on the basis of NMR and HRESIMS analyses. The absolute configurations of compounds …
Nettet14. jan. 2024 · Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. Properties Articles 72 Spectrum Names Hoechst 33342 Biological Activity Chemical & Physical Properties MSDS Hoechst 33342 MSDS (Chinese) Toxicological Information Safety Information Synthetic Route Previous 1/3 Next … first term exam 2079NettetThe Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. campers with loft sleepingNettetIt is quite toxic to some cell types and potentiates may toxins bacuse it shares xenobiotic drug pumps. The BEST way to use Hoechst33342 dye is to make up a stock in DMSO … campers with pop out bedsNettetCell cycle specific toxicity of the Hoechst 33342 stain in untreated or irradiated murine tumor cells. Hoechst 33342 stain has been used previously in conjunction with … first terminal examination 2076 mathNettet6. mai 2024 · Answer Hoechst is minimally toxic to cells especially when used at very low concentrations. It is the preferred dye for staining live cells because of its lower … first terminal university level qualificationNettetToxicity Toxic, harmful Health Hazards Wear PPE at all times, handle with glove. Potential Hazards CNS and Liver; harmful by skin absorption, irritant mutagen. Carcinogenicity: No significant acute toxicological data identified AnaSpec Inc. 34801 Campus Drive, Fremont, CA 94555 Toll-Free: 800-452-5530 • Tel: 510-791-9560 • Fax: … first term holidays 2022NettetChemical structure for Hoechst 33342 *Ultrapure Grade* *CAS 23491-52-3* Figure 7. Dose-dependent toxicity of diverse NPs towards HUVECs as measured by CDS assay. The HUVECs in the 96-well plate were treated with 0–100 μg/ml of SiNP, SPION or CNTCOOH NPs for 24 hours. campers with sliding doors