Genome analyses of single human oocytes

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August undefined, 2024

WebGenome Analyses of Single Human Oocytes. Author links open overlay panel Yu Hou 1 6, Wei Fan 1 4 6, Liying Yan 1 6, Rong Li 1, Ying Lian 1, Jin Huang 1, Jinsen Li 1, Liya Xu 1, Fuchou Tang 1 5, X. Sunney Xie 1 2, Jie Qiao 1 3. ... Whole-genome amplification and sequencing of single human oocytes using MALBAC method ... WebHere, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing …

Genome analyses of single human oocytes Yu Hou, Ph.D.

WebApr 23, 2024 · To the best of our knowledge, this is the first study to use multi-omics single-cell analysis to compare the transcriptomes, DNA methylomes and genomic CNVs of IVT and IVV matured human oocytes. Single-cell analyses of differences in the transcriptomic profiles of oocytes derived from women with and without PCOS and at different stages of ... WebJan 30, 2014 · Single-cell analyses of human embryos following in vitro fertilization (IVF) ... Hou Y, Fan W, Yan L, Li R, Lian Y, et al. (2013) Genome analyses of single human oocytes. Cell 155: 1492–1506. View Article Google Scholar 46. Conrad DF, Keebler JE, DePristo MA, Lindsay SJ, Zhang Y, et al. (2011) Variation in genome-wide mutation … kiss band young pictures

Uncovering hidden mitochondrial mutations in single cells

WebApr 4, 2024 · Unbiased analysis of the whole mutational landscape of mtDNA in single human oocytes. (A) Circular plots showing the distribution of SNVs in the mitochondrial genome of hOO_1.The innermost circle (grey) shows the depth of reads of all detected UMI groups in linear scale as indicated by the scale bar in the center. WebSep 2, 2024 · Developing female human germ cells undergo genome-wide epigenetic reprogramming, but de novo DNA methylation dynamics and their interplay with chromatin states and transcriptional activation in developing oocytes is poorly understood. Here, we developed a single-cell multi-omics sequencing method, s … WebMay 22, 2013 · Pathway Analysis of Single Human Oocytes and Blastocysts. We analysed components of 10 different pathways that were highly represented (P<0.05; q<0.22) in our individual human oocyte and … lysol healthing commercial

Large-scale analysis of de novo mutations identifies risk genes for ...

Genome analyses of single human oocytes Yu Hou, Ph.D.

WebHere, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with … WebJan 8, 2024 · A total of 149 human oocytes were collected at the Centre for Reproductive and Genetic Health (CRGH), London (UK), after obtaining appropriate research consent. The oocytes were collected between the years 2015 and 2024. The oocytes were donated by 42 women between the ages of 25 and 44 years (mean ± SD of maternal age 35 ± 3.4 … kiss bassmachineWebApr 13, 2024 · A human blastocyst-like synthetic embryo called blastoid showing the presence of an enveloping layer of extra-embryonic cells, a blastocoel-like cavity, … kiss beach bum comic nail strips

"WebApr 14, 2024 · Dolosigranulum pigrum phylogenetic and core genome analysis. We first analyzed the genetic diversity of 21 D. pigrum whole genome sequences available (n = 7 from NCBI and n = 14 from in-house D ... " - Genome analyses of single human oocytes

Genome analyses of single human oocytes

Study finds mitochondrial mutations in single cells

Web6 hours ago · Using third-generation nanopore sequencing technology, the researchers have characterized mtDNA heteroplasmy in single cells and described the genetic features of … WebAug 30, 2024 · Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation. ... 41 against build hg38 of the human ...

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WebSep 5, 2024 · Genome analyses of single human oocytes. Cell. 2013; 155: 1492-1506. Abstract; Full Text; Full Text PDF; PubMed; Scopus (235) Google Scholar, Lu et al., 2012. Lu S. Zong C. Fan W. ... analyses by single sperm or oocyte sequencing are limited in throughput and to a few hundred cells at the most, and as such could miss out on rare …

Web14 minutes ago · The genetic maps of mtDNA in individual human oocytes (immature eggs) and blastoids (synthetic embryos derived from stem cells) have recently been quantitatively described by an international team ... Web14 minutes ago · The genetic maps of mtDNA in individual human oocytes (immature eggs) and blastoids (synthetic embryos derived from stem cells) have recently been …

WebFeb 25, 2024 · Genome-wide expression studies in human and animal models support this hypothesis, ... Mallol A, Elosua-Bayes M, Lorden P, Ruiz S, Zambelli F, Heyn H, Vassena R, Payer B. Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age. Aging Cell. 2024;20(5):e13360. WebAug 30, 2024 · Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation. ...

WebJan 5, 2024 · Here, we investigate an alternative approach, namely, de novo reconstruction of haploid human oocytes by recycling first polar body (PB1) genomes via nuclear transfer into donor cytoplasm (Gurdon, ... Genome analyses of single human oocytes. Cell, 155 (2013), pp. 1492-1506. View PDF View article View in Scopus Google Scholar. Huang …

WebGenome Analyses of Single Human Oocytes. Author links open overlay panel Yu Hou 1 6, Wei Fan 1 4 6, Liying Yan 1 6, Rong Li 1, Ying Lian 1, Jin Huang 1, Jinsen Li 1, Liya … lysol health risksWebApr 4, 2024 · Unbiased analysis of the whole mutational landscape of mtDNA in single human oocytes. (A) ... kiss band youtubeWebSingle-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole … kiss baseball capWebDec 19, 2013 · In 2013, Tang and Qiao's group [139] used single-cell WGS to reveal the morphological and dynamic changes of chromosomes during oocyte development, and … lysol healthing i spot tvWebHere, we demonstrate genome analyses of single human oocytes using multiple annealing and looping-based amplification cycle (MALBAC)-based sequencing technology. By sequencing the triads of the first and second polar bodies (PB1 and PB2) and the oocyte pronuclei from same female egg donors, we phase the genomes of these donors with … lysol healthWebMay 3, 2024 · Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, et al. Genome analyses of single human oocytes. Cell. 2013;155(7):1492–506. Article CAS Google Scholar Yan ZQ, Zhu XH, Wang YQ, et al. scHaplotyper: haplotype construction and visualization for genetic diagnosis using single cell DNA sequencing data. lysol healthcareWebJie Qiao and colleagues describe how high-throughput sequencing of oocyte polar bodies could enhance preimplantation genomic screening in IVF. lysol healthy habits